Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria.
نویسندگان
چکیده
We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance gene mecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.
منابع مشابه
A Low-aspect-ratio, Roll-to-roll Hot Embossed Inertial Microfluidic Sorter
This work presents a low-aspect-ratio (LAR) inertial microfluidic design compatible with roll-to-roll (R2R) hot embossing for size-based cell or microparticle separation in a low-cost and disposable format. The device consists of two segments of LAR channels in which inertial lift forces are used to differentiate lateral positions based on size for separation downstream. Separation of 18μm and ...
متن کاملAntibiotic Resistance Pattern and Detection of mecA Gene in Staphylococcus aureus Isolated from Iranian Hamburger Samples
Background: Among the bacteria that cause food poisoning, Staphylococcus aureus is one of the most common causes of food poisoning worldwide. The aim of this study was to investigate the presence of S. aureus strains in Iranian hamburgers, analysis of their antibiotic resistance pattern, and molecular detection of mecA gene in isolated strains. Methods: A total of 100 Iranian handmade (traditi...
متن کاملDetection of Gene Encoding Enterotoxin A in Staphylococcus aureus Isolated from Cream Pastries
Background: Staphylococcus aureus has always been known as an important kind of bacteria causing food poisoning. This study was carried out to determine S. aureus contamination in cream pastries collected from popular confectioneries in Hamedan, Iran; also, presence of the gene encoding Staphylococcal Enterotoxin A (SEA) was studied in the isolates. Methods: During April to October 2014 (as ho...
متن کاملEffects of Preheat Supply on Embossed Pattern Depth in Roll-to-Roll Process
In this study, we examined the sensitivity of embossed pattern depth to preheat supply and cooling, and also tested how pattern type and density affect the embossed depth. The main factors such as roller temperature, roller speed, and applied force that mostly affect embossed pattern qualities of roll-to-roll hot embossing were determined based on the response surface methodology. Eight conditi...
متن کاملPrediction of Work Roll Initial Crown according to Desired Strip Profile in Hot Rolling
The final hot strip profile is a superposition of the roll initial crown, the roll bending and flattening crown, the roll wearing crown and the roll thermal crown. In this research, linear regression models are proposed to predict the roll force, the roll wearing crown and the roll thermal crown using experimental data provided by Mobarake Steel Complex (MSC). The short beam theory based on ela...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Lab on a chip
دوره 12 2 شماره
صفحات -
تاریخ انتشار 2012